Recombinant:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Sterility:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Saline:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Software:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Imaging:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Microscopy:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
Fluorescence:Article Title: 3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.
Article Snippet: .. GENT or RESOURCE SOURCE IDENTIFIER icals, peptides, and recombinant proteins ose VWR Cat#R101175P ctose Sigma Cat#G0750 nose Sigma Cat#R0250 (Continued on next page) STAR Protocols 2, 100525, June 18, 2021 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Dropout Base Medium (DOB) MP Biomedicals Cat114025012-CF SC Amino Acid Mixture (Hopkins mixture) MP Biomedicals Cat#114400022 Yeast extract powder Sigma Cat#Y1625 Bacto Peptone BD Biosciences Cat#211677 Bacto Agar BD Biosciences Cat#214010 Adenine Sigma Cat#A8626-100G a-factor Biotem alpha-factor Hydroxyurea Sigma Cat#H8627 Methyl methanesulfonate Sigma Cat#129925 H2O sterile Sigma Cat#W3500 Phosphate buffered saline (PBS) 103 Sigma Cat#P7059-1L Concanavalin A Sigma Cat# L7647-25MG Paraformaldehyde 16% VWR/Alfa Aesar Cat# 43368.9M Software and algorithms Huygens Professional Scientific Volume Imaging https://svi.nl/Huygens-Professional Image J/Fiji https://bioviz.org/ https://fiji.sc/ PointPicker plugin for Fiji Philippe Thévenaz http://bigwww.epfl.ch/thevenaz/pointpicker/ Imaris Oxford Instruments https://imaris.oxinst.com/ Other 50 mL Conical tube with filter cap CELLSTAR CELLreactor Greiner Bio-One Cat#227245 Grace Bio-Labs SecureSeal Sigma Cat#GBL654006-100EA Microscope cover glasses 1.5H 18318 mm Zeiss Cat#019030091 Microscope slides SuperFrost Menzel Gläser Thermo Scientific Cat#AG00008032E01FST20 Microscope Zeiss Axio Imager Z2 Objective lens alpha Plan-Apochromat 1.46 oil DIC M27 Zeiss Cat#420792-9800 High sensitivity camera sCMOS ORCA-Flash 4.0 LT+ Hamamatsu Cat# C11440-42U30 Fluorescence excitation light source (widefield) Excelitas X-Cite 120 LED Excitation and emission filters (eGFP) Zeiss BP 470/40, FT 495, Em. .. BP 525/50 Excitation and emission filters (mCherry) Zeiss FS45 Carl Zeiss Ex.
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